Fluorosis Research And Referral Laboratory

The Fluorosis Research and Referral Laboratory (FRRL) provide location specific and meaningful strategies to mitigate the fluorosis and it’s linked disorders. The center was identified as a Center of Excellence by the Academy vide reference No. SDUAHER/KLR/ADMN/330/2020-21 dated 14.10.2020.

Programs

Functional MoU

MoU titled "Fluoride related research and IPR related activities" pertaining to Fluorosis research was signed between Sri Devaraj Urs Academy of Higher Education and Research, Kolar and Acharya Institutes, Bangalore on 12- 07- 2017.

Guest Lecture

"Fluorosis" by Dr. Bapuji M and Dr. Krishna Rao on 12 – 04- 2017.

State Level CME

"Minerals in health and disease with special emphasis on Fluoride" on 12 – 05 – 2017.

National Webinar

"Fluorosis and its Global Perspective" was conducted on 06.03.2021 to create awareness on fluorosis and disseminate the information.

New Knowledge Generated: Academic researchers and advanced practitioners deliberated on fluoride containing dental products like fluoride gel, fluoride varnish, mouth rinses and fluoride containing tooth pastes also contain high amount of fluoride but adolescents and professional’s knowledge on this product and their fluoride concentration is very low. Many natural adsorbents from various trees and animal sources have been tried as defluoridation agents.

Seeds of the drumstick, tamarind seeds, tea ash, and egg shell powder are few among them. This new knowledge generated can help in establishing effective ways to mitigate fluorosis.

Goals

Short Term goals
  1. Establishment of the Laboratory
  2. Taking up research projects in association with other departments
Mid Term goals  
  1. Procurement of funds from SDUAHER and extramural funds
  2. Conduct of CME/workshops/guest lectures to create awareness
Long Term goals
  1. Conduct of camps in and around Kolar district and thus get patients for symptoms of fluorosis to be managed.
  2. Preparation of formulations with readily available natural resources to ease symptoms/prevent fluorosis
  3. To seek a tie up with Government of Karnataka/NGO’s/ other Universities for furthering fluoride research
Future plans        
  1. To establish research at the molecular level to find out effect of fluoride at the gene level by taking up studies on Genomics/Proteomics
  2. To develop in house cell lines and initiate research work on cell culture
  3. Since, Kolar district is mainly dependent on agriculture and rearing of Life stock the services of this lab can be extended to these levels of society also.

Research & Publications

Instrumentation at Fluorosis Referral & Research Laboratory for fluoride analysis

Publications
Sl. No. Project title Investigators Details Journal
1 Effect of Moringa oleifera leaves on hematological profile of fluorosis affected rats Pravallika P MS Vinutha Shankar A Hemalatha KN Shashidhar SDUAHER article 2022 Bioinformation 18(1): 14-18 (2022)
2 Changes in diabetic and renal profile of people exposed to fluoride in south India KN Shashidhar, Munilakshmi U Sai Deepika R Deena Mendez Raveesha A SDUAHER article 2022 Bioinformation 2022;18(9): 820-824
3 Mysterious Mineral: Fluoride Sai Deepika Shashidhar K N Sreeramulu PhD review article 2019 J. Evolution Med. Dent. Sci. 2019;8 (48); 3617- 3623
4 Estimation of Serum Fluoride and Renal Parameters in Diabetic Nephropathy- A Facility Based Observational Case Control Study Mohan Krishna Munilakshmi. U Saideepika. R Shashidhar K.N Bhuneswar Yadav STS – ICMR 2020 article Biomedical & Pharmacology Journal, March 2020;13(2):571-576
5 A Cross-Sectional Study of Serum and Urine Fluoride in Diabetes in Fluoride Exposed Population Sai Deepika Shashidhar K N Raveesha A Muninarayana C PhD article 2021` J Evolution Med Dent Sci 2021; 10(11):798- 801
6 Estimation of Fluoride and Sirtuin1 in Patients with Diabetic Nephropathy in Kolar District of Karnataka, India   Saideepika. R Shashidhar K.N Raveesha A Muninarayana C PhD article 2021   J Lab Physicians. 2022; 14(1): 57–64
7 Serum Fluoride Levels As A Biomarker In Pre- eclamptic Women: A Case –Control Tertiary Care Hospital Based Study Changalvala K Prabhavathi K KV Venkateshu SR Sheela, R Kalyani CV Raghuveer Kiranmayee P PhD article 2021   Fluoride 53(31):425-435
8 Chronic Fluorosis- A Disease of concern Deena Mendez Shashidhar KN Review International Journal of Current Research and Review. 2021;13(17):30-36.
9 Significance of Copper, Zinc, Selenium and Fluoride in Squamous Cell Carcinoma of Cervix- A Pilot Study GS Meghana R Kalyani MESumathi SR Sheela Student Project JCDR 2019 13(5): EC17-EC20

Research Projects

In House Fluorosis Projects Completed (09)

Sl. No. Topic Investigators Details
  1.  
 Estimation of Fluoride and Sirituin 1 in Patients with diabetic Nephropathy in Kolar District of Karnataka, India Mrs. Sai Deepika Dr. K.N. Shashidhar Dr. C. Muninarayana. C Dr. A Raesveha Ph.D Thesis Work  
  1.  
 Correlation of serum and urine fluoride with anemia and maternal perinatal outcome in pregnancy. Dr. Sneha Singh Dr. Munikrishna Dr. Shashidhar.K.N PG Dissertation  
  1.  
 Association of plasma fluoride levels with occurrence of pre-diabetes in three endemic villages of Kolar district. Dr. Sumathi.M.E Dr. V. Lakshmaiah Dr. Shashidhar.K.N Dr. Hariprasad.S Dr. Ravishankar.S SDUAHER funded  
  1.  
 Effect of moringa olifera leaves on hematological profile and histomorphology of brain, liver, spleen and kidneys of fluorosis affected rats Mrs. Pravallika P Dr. Vinutha Shankar Dr. Shashidhar.K.N Dr. Hemalatha A SDUAHER funded  
  1.  
 Knowledge and awareness about fluorosis in rural population of Kolar district Dr. Sumathi M E Ms Sangeetha P Project Completed  
  1.  
 Impact of increased fluoride intake on function of Thyroid hormones in Kolar district- An area endemic for fluorosis Ms. Nagina. R Dr. Deena C Mendez   MSc. MLT Clinical Biochemistry Project
  1.  
 Association of Vitamin D binding protein in Diabetic Nephropathy: A cross sectional analytical study Mr. Bhuneshwar Yadav Dr. Shashidhar KN Dr. C. Muninarayana. C Dr. A Raveesha Ph.D Thesis Work  
  1.  
 To find association of Fluoride in Diabetes Type 2 and its Microvascular Complications. A Tertiary Care Rural Hospital Case Control Study Dr. Shashidhar K.N Dr. Deena Mendez Dr. Munilakshmi .U Dr. Raveesha Dr. Sangeetha. T SDUAHER funded  
  1.  
 Serum biomarkers, serum fluoride levels estimation with histopathological changes in placenta of pre-eclamptic women Mrs. Krishnaveni Dr. C.V. Raghuveer Dr. Sheela S.R. Dr. Venkateshu K.V Dr. Prabhavathi. K Ph.D Thesis Work  

In House Fluorosis Projects Ongoing (04)

Sl. No. Topic Investigators Details
1 Histomorphological change and glutamate levels in Fluoride and Arsenic induced injury to Hippocampus and cerebral cortex in Wistar rats and ameliorating effects of Ginkgobiloba Mr. Suresh.T Dr. C.V. Raghuveer Dr. Venkateshu K.V Dr. Kiranmayee P Ph.D Thesis Work  
2 Role of Fluoride and oxidative stress parameters in senile cataract: A tertiary care hospital study Dr. Sangeetha. T Dr. Shashidhar KN Dr. Suresh TN Ph.D Thesis Work  
3 Influence of Fluorosis on Genetic predisposition due to single nucleotide Polymorphism of LOX1 gene in pseudoexfoliation syndrome Dr. Chaitra MC Dr. Rashmi G Dr. Sumathi M E Dr. Sharath B Dr. SM Azeem Mohiyuddin Mrs. Apoorva HM SDUAHER funded  
4 Clinical and laboratory profile in paediatric patients with bicytopenia or pancytopenia in a tertiary care centre in kolar- A cross sectional study Dr. Sai Teja Dr. KNV Prasad Dr. Shashidhar KN   MD Dissertation  

Extramural Funded Project

Sl. No. Topic Investigators Details
1 Identification of Sirtuin1 Gene polymorphism associated with fluorosis and type 2 diabetes mellitus PI: Dr. Shashidhar KN Co- PI: Dr. R. Sai Deepika RA: Mr. Meghanath GH DHR- ICMR funded Rs. 19,10,000 total fundung for 3 years  

CONSULTANCY PROJECT

EXTENSION ACTIVITIES

Faculties

Dr. Shashidhar K N Professor & Vice Principal
Dr. R. Sai Deepika Senior Resident of Biochemistry
Dr. Munilakshmi U Assistant Professor